Intact liposome-based targeted nanoparticle delivery systems (NDS) are immobilized by nonselective binding and characterized by scanning probe microscopy (SPM) in a fluid imaging environment. The size, size distribution, functionality, and stability of an NDS with a payload consisting of a super-paramagnetic iron-oxide (SPIO) contrast agent for magnetic resonance imaging (MRI) are determined. SPM results are combined with information obtained by more familiar techniques such as superconducting quantum interference device (SQUID) magnetometry, dynamic light scattering (DLS), and electron microscopy. By integrating the methods presented in this work during NDS formulation and manufacturing, size-dependent statistical properties of the complex can be obtained and structure-function relationship of individual, multi-component nanoscale entities can be assessed in a reliable and reproducible manner.
Pub Type: Journals
contrast agent, liposome, magnetic resonance imaging, nanoparticle delivery system, scanning probe microscopy