Protein adsorption mediates biological responses to materials. When a biodegradable polymer scaffold is placed in aqueous, physiological medium, proteins adsorb onto the scaffold surface. The total amount, composition and structure of the adsorbed protein layer can change over time and could influence cell adhesion and proliferation. Thus, we have tested the hypothesis that cell response to a polymeric scaffold is affected by the amount of time that the polymer has been incubated in physiological, serum-containing medium and the amount of protein adsorbed to the scaffold. Previous work has shown that pre-ageing of polymer films in serum-containing cell culture medium enhanced osteoblast adhesion and proliferation . Towards tissue engineering of bone, we examined the effect of pre-aging time of three-dimensional (3D) polymer scaffolds in serum-containing medium on 1) amount of protein adsorbed and 2) osteoblast attachment and proliferation.
Conference Dates: April 13-16, 2011
Conference Location: Orlando, FL
Conference Title: Society for Biomaterials
Pub Type: Conferences
polymer scaffold, osteoblast, cell adhesion, cell proliferation, protein adsorption