Almost all nanofluidic devices for biopolymer analysis have been limited by one or two confining structural dimen-sions or the application of external forces for biopolymer manipulation, which has restricted the scope of related lab-on-a-chip technology. Here, a nanofluidic channel with a three dimensional (3D) staircase depth profile across its width was used to control and measure the behavior of individual DNA molecules, which descended the confining staircase via entropophoresis effected by interactions between the DNA molecules and the nanofluidic step edges. Thus, the nanofabricated staircase enabled complex structural control over DNA transport and conformation without the applica-tion of external forces.
Proceedings Title: The 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Conference Dates: October 3-7, 2010
Conference Location: Gaithersburg, MD
Pub Type: Conferences
3D, DNA, Entropophoresis, Nanofluidics, Polymer, Separation