Modifications to the NIST Reference Measurement Procedure (RMP) for the Determination of Serum Glucose by Isotope Dilution Gas Chromatography/ Mass Spectrometry
Jocelyn L. Prendergast, Lorna T. Sniegoski, Michael J. Welch, Karen W. Phinney
NIST has been providing a Standard Reference Material (SRM) for glucose in human serum for many years to support accuracy in health care measurements. The definitive method (DM), now known as the reference measurement procedure (RMP), for the analysis of glucose in serum was originally published in 1982 by NIST. Over the years the method has been subject to a number of modifications in order to adapt to newer technologies and simplify sample preparation. Recently this method has undergone the elimination of a deionization step, which is the most significant change made to date. In addition, high performance capillary columns have replaced the SCOT columns used in the original work and better electronics have enabled bench top quadrupole mass spectrometers to perform measurements with precisions that approach those only attainable in the past with large, complex magnetic sector mass spectrometers. We discuss here an adaptation of the method associated with serum glucose measurements using a modified isotope-dilution gas chromatography / mass spectrometry (ID-GC/MS) method. The glucose was deproteinized with ethanol and derivatized to form α-D-glucofuranose cyclic 1,2:3,5-bis(butylboronate)-6-acetate (glucose BBA). The method uses uniformly labeled (13C) glucose as the internal standard and samples were analyzed by GC/MS. This modified method demonstrates both good precision and accuracy in addition to reducing the sample preparation time by a day and a half. NIST has used this modified method to certify the concentrations of glucose in SRM 965b, Glucose in Frozen Human Serum and SRM 1950, Metabolites in Human Plasma.
, Sniegoski, L.
, Welch, M.
and Phinney, K.
Modifications to the NIST Reference Measurement Procedure (RMP) for the Determination of Serum Glucose by Isotope Dilution Gas Chromatography/ Mass Spectrometry, Analytical and Bioanalytical Chemistry, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=904017
(Accessed June 4, 2023)