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Methods for the Separation and Quantification of Arsenic Species in SRM 2669: Arsenic Species in Frozen Human Urine

Published

Author(s)

William C. Davis, Rolf L. Zeisler, John R. Sieber, Lee L. Yu

Abstract

Two independent liquid chromatography inductively coupled plasma mass spectrometry (LC/ICP-MS) methods for the separation of arsenic species in urine have been developed with quantification by standard additions. Seven arsenic species have been quantified in a new NIST frozen human urine Standard Reference Material (SRM) 2669 (Arsenic Species in Frozen Human Urine), Level 1 and Level 2. The species measured were: arsenite (As(III)), arsenate (As(V)), monomethylarsonate (MMA), dimethylarsinate (DMA) arsenobetaine (AB), arsenocholine (AC), and trimethylarsine oxide (TMAO). The purity of each arsenic standard used for quantification was measured as well as the arsenic species impurities determined in each standard. Analytical method limits of detection (LD) for the various species in both methods ranged from 0.2 μg L-1 to 0.8 μg L-1 as arsenic. Measurements of the arsenic species three years after initial production of the SRM demonstrate the stability of the arsenic species in the urine reference material. The results demonstrate that LC/ICP-MS is a sensitive, reproducible, and accurate technique for the determination of low-level arsenic species in urine.
Citation
Analytical and Bioanalytical Chemistry
Volume
396
Issue
8

Keywords

speciation, arsenic, urine, ICP-MS

Citation

Davis, W. , Zeisler, R. , Sieber, J. and Yu, L. (2010), Methods for the Separation and Quantification of Arsenic Species in SRM 2669: Arsenic Species in Frozen Human Urine, Analytical and Bioanalytical Chemistry, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=903868 (Accessed June 3, 2023)
Created March 26, 2010, Updated February 19, 2017