Method development for the determination of seven ginsenosides in three Panax Ginseng Standard Reference Materials via liquid chromatography with tandem mass spectrometry

Published

October 18, 2022

Author(s)

Hugh Hayes, Walter Brent Wilson, Kate Rimmer

Abstract

A new liquid chromatography-tandem mass spectrometry (LC–MS/MS) method was developed for the analysis of ginsenosides in three Panax ginseng reference materials (RMs). Extraction procedures were optimized to recover neutral and malonyl-ginsenosides using a methanol–water extraction under basic conditions. Optimized mass fragmentation transitions were obtained for the development of a multiple reaction monitoring (MRM) detection method with electrospray ionization in negative and positive ion mode. Mass fraction values were determined for ginsenosides Rb1, Rb2, Rc, Rd, Re, Rf, and Rg1 in the three ginseng materials (rhizomes, extract, and an oral dosage form). Quantitation of these seven compounds was accomplished with 4-methylestradiol and SRM 3389 Ginsenoside Calibration Solution serving as an internal standard (IS) and calibration standards, respectively. Mass fraction values for the seven ginsenosides ranged from 1.27 mg/g to 21.42 mg/g, 3.25 mg/g to 35.81 mg/g, and 0.56 mg/g to 2.51 mg/g for SRM 3384, SRM 3385, and RM 8664, respectively.

Citation

Analytical and Bioanalytical Chemistry

Pub Type

Journals

Download Paper

DOI Link

Keywords

Ginseng, Liquid chromatography-tandem mass spectrometry, reference materials, natural products, method development, dietary supplementation

Citation

Hayes, H. , Wilson, W. and Rimmer, K. (2022), Method development for the determination of seven ginsenosides in three Panax Ginseng Standard Reference Materials via liquid chromatography with tandem mass spectrometry, Analytical and Bioanalytical Chemistry, [online], https://doi.org/10.1007/s00216-022-04378-9 (Accessed April 26, 2024)