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MALDI of Layered Polymer Films

Published

Author(s)

Barry J. Bauer, Kathleen M. Flynn, B D. Vogt

Abstract

A layered preparation method is introduced that produces structured MALDI targets with well-defined phase sizes and compositions. Tri-a-naphthyl benzene is a glassy matrix that forms smooth films. It is layered with polystyrenes or polyethylene glycol dissolved in selective solvents that do not affect previously applied layers. Both hydrogenous and deuterated polystyrenes are used in the layering process so that they can be distinguished by MALDI. The results are consistent with the solvent-free sample preparation method and small angle neutron scattering studies, which show that molecular dispersion of individual polymer molecules is not necessary for strong signals. Individual polymer molecules in micron sized layers produce varied MALDI signals depending on their location within the aggregates. This suggests a possible mechanism for biasing in MALDI due to unequal distributions of polymers throughout the sample.
Proceedings Title
American Chemical Society. Division of Polymeric Materials: Science and Engineering Meeting| | Proceedings of the ACS Division of Polymeric Materials:Science & Engineering|ACS
Volume
90
Conference Dates
March 28-29, 2003
Conference Location
Undefined
Conference Title
American Chemical Society Polymeric Materials Science and Engineering Division Preprints

Keywords

biasing, layer, MALDI, matrix, polymer

Citation

Bauer, B. , Flynn, K. and Vogt, B. (2004), MALDI of Layered Polymer Films, American Chemical Society. Division of Polymeric Materials: Science and Engineering Meeting| | Proceedings of the ACS Division of Polymeric Materials:Science & Engineering|ACS, Undefined, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=852287 (Accessed April 14, 2024)
Created February 29, 2004, Updated October 12, 2021