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Liquid Chromatography: Introduction and Instrumentation

Published

Author(s)

Lane C. Sander

Abstract

Chromatography is an example of a process by which a mixture is separated into at least two fractions with different compositions. This presentation will provide a brief overview of the practice of liquid chromatography (LC), with an emphasis on chromatographic instrumentation. Instrumentation often differs significantly among manufacturers, but the principles of operation are the same. Analytical methods usually can be transferred from one instrument to another, as long as the basic requirements are maintained. Some variability is to be expected based on differences in instrumentation, but the changes in performance can often be compensated by adjusting operational parameters. The presentation includes a brief historical perspective and schematic illustration of column chromatography. The design and operation of modern chromatographic instrumentation is described, including individual components. The principle of operation of a reciprocating piston pump with inlet and outlet check valves is illustrated by an animated sequence. An overview of LC detectors is provided to include absorbance detectors (single wavelength, variable wavelength, and diode array), and detectors based on fluorescence, refractive index, evaporative light scattering, electrochemical, or mass spectrometry. A laboratory video is included to demonstrate the startup procedure and operation for a typical liquid chromatograph.
Citation
Journal of Research (NIST JRES) -
Volume
122

Keywords

liquid chromatography, instrumentation, LC theory, chromatographic pumps, operation, check valves

Citation

Sander, L. (2017), Liquid Chromatography: Introduction and Instrumentation, Journal of Research (NIST JRES), National Institute of Standards and Technology, Gaithersburg, MD, [online], https://doi.org/10.6028/jres.122.001 (Accessed May 22, 2024)

Issues

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Created January 12, 2017, Updated January 27, 2020