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Liquid Chromatography Column Theory and Technology



Lane C. Sander


Column selection is probably the single most important aspect of method development that will directly affect measurement accuracy and precision of liquid chromatographic (LC) measurements. An understanding of physical and chemical properties of LC columns will greatly assist the development and optimization of robust analytical methods. This presentation will describe aspects of the preparation, characterization, and use of different types of liquid chromatographic columns, to facilitate informed selection of columns for the development of new methods. Topics include: substrates, synthesis of bonded stationary phases, spectroscopic approaches to characterize covalently modified surfaces, chromatographic retention mechanisms for normal-phase and reversed-phase separations, and properties of the most common types of commercially available columns, including newly developed core-shell technologies.
Journal of Research (NIST JRES) -


liquid chromatography, columns, stationary phase, synthesis, characterization, covalently modified surfaces, retention mechanism


Sander, L. (2017), Liquid Chromatography Column Theory and Technology, Journal of Research (NIST JRES), National Institute of Standards and Technology, Gaithersburg, MD, [online], (Accessed July 22, 2024)


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Created March 8, 2017, Updated January 27, 2020