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Length Dependent Uptake of DNA-Wrapped Single Walled Carbon Nanotubes

Published

Author(s)

Matthew Becker, Jeffrey Fagan, Nathan D. Gallant, Barry J. Bauer, Vardhan Bajpai, Erik K. Hobbie, Silvia H. De Paoli Lacerda, Kalman D. Migler, J P. Jakupciak

Abstract

DNA-wrapped single wall carbon nanotubes (SWNTs) with broad length distributions are shown to reduce the viability of IMR90 primary human lung fibroblast cells above the (23 to 25) ug/mL concentration regime. Furthermore using well-defined length fractions of SWNT separated by size exclusion chromatography and concentrated via forced air dialysis we identified a threshold for cell uptake near 189 17 nm. The identification of a length threshold for SWNT toxicity suggests that shorter tubes are more toxic in vitro while longer SWNTs are not consumed by IMR90 cells. These results will provide important insight to researchers and manufacturers where exposure to SWNT is a regulatory, environmental or safety concern.
Citation
Journal of the American Chemical Society
Volume
19

Keywords

AFM, carbon nanotube, IMR90, in vitro, preferential uptake, SWNT, TEM, toxicity, Wst-1

Citation

Becker, M. , Fagan, J. , Gallant, N. , Bauer, B. , Bajpai, V. , Hobbie, E. , De Paoli Lacerda, S. , Migler, K. and Jakupciak, J. (2007), Length Dependent Uptake of DNA-Wrapped Single Walled Carbon Nanotubes, Journal of the American Chemical Society, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=852685 (Accessed April 23, 2024)
Created December 31, 2006, Updated October 12, 2021