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Isotope Dilution Liquid Chromatography-Tandem Mass Spectrometry for Quantitative Amino Acid Analysis

Published

Author(s)

David M. Bunk, Mark S. Lowenthal

Abstract

The role of amino acid analysis in bioanalysis has changed from a qualitative to a quantitative technique. With the discovery of both electrospray ionization and matrix-assisted laser desorption ionization in the early 1990s, the use of amino acid analysis for qualitative analysis of proteins and peptides has been replaced by mass spectrometry. Accurate measurement of the relative molecular masses of proteins and peptides, peptide mapping, and sequencing by tandem mass spectrometry provide significantly better qualitative information than can be achieved from amino acid analysis. At NIST, amino acid analysis is used to assign concentration values to protein and peptide standard reference materials (SRMs) which, subsequently, will be used in the calibration of a wide variety of protein and peptide assays, such as those used in clinical diagnostics. It is critical that the amino acid analysis method used at NIST for assigning concentration values in SRM deliver the highest accuracy and precision possible. Therefore, we have developed an amino acid analysis method that uses isotope dilution LC-MS/MS - the analytical technique routinely used at NIST to certify analyte concentrations in SRMs for a wide variety of analytes. We present here our most recent method for the quantification of amino acids using isotope dilution LC-MS/MS.
Citation
Amino Acid Analysis: Methods and Protocols, Second Edition
Volume
2030
Publisher Info
Springer Science Business Media, New York, NY

Keywords

Isotope dilution, Liquid chromatography, Tandem mass spectrometry, Quantification, Amino acid analysis

Citation

Bunk, D. and Lowenthal, M. (2019), Isotope Dilution Liquid Chromatography-Tandem Mass Spectrometry for Quantitative Amino Acid Analysis, Amino Acid Analysis: Methods and Protocols, Second Edition, Springer Science Business Media, New York, NY, [online], https://doi.org/10.1007/978-1-4939-9639-1_12 (Accessed October 3, 2022)
Created June 2, 2019, Updated March 1, 2021