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PUBLICATIONS

Isotope Dilution Liquid Chromatography - Mass Spectrometry Methods for Fat- and Water-Soluble Vitamins in Nutritional Formulations

Published

Author(s)

Karen W. Phinney, Catherine A. Rimmer, Jeanice M. Brown Thomas, Lane C. Sander, Katherine E. Sharpless, Stephen A. Wise

Abstract

Vitamins are essential to human health, and dietary supplements containing vitamins are widely used by individuals hoping to ensure they have adequate intake of these important nutrients. Measurement of vitamins in nutritional formulations is necessary to monitor regulatory compliance and in studies examining the nutrient intake of specific populations. Liquid chromatographic methods, primarily with UV absorbance detection, are well established for both fat- and water-soluble measurements, but they do have limitations for certain analytes and may suffer from a lack of specificity in complex matrices. Liquid chromatography-mass spectrometry (LC-MS) provides both sensitivity and specificity for the determination of vitamins in these matrices, and simultaneous analysis of multiple vitamins in a single analysis is often possible. In this work, LC-MS methods were developed for both fat- and water-soluble vitamins and applied to the measurement of these analytes in two NIST Standard Reference Materials. When possible, stable isotope labeled internal standards were employed for quantification.
Citation
Analytical Chemistry
Volume
83
Pub Type
Journals

Download Paper

DOI Link

Keywords

dietary supplement, isotope dilution, liquid chromatography, mass spectrometry, vitamin
Chemistry and Analytical chemistry

Citation

Phinney, K. , Rimmer, C. , Brown, J. , Sander, L. , Sharpless, K. and Wise, S. (2011), Isotope Dilution Liquid Chromatography - Mass Spectrometry Methods for Fat- and Water-Soluble Vitamins in Nutritional Formulations, Analytical Chemistry, [online], https://doi.org/10.1021/ac402689t (Accessed October 9, 2025)

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Created November 24, 2011, Updated November 10, 2018
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