Isotope Dilution Liquid Chromatography - Mass Spectrometry Methods for Fat- and Water-Soluble Vitamins in Nutritional Formulations

Published

November 24, 2011

Author(s)

Karen W. Phinney, Catherine A. Rimmer, Jeanice M. Brown Thomas, Lane C. Sander, Katherine E. Sharpless, Stephen A. Wise

Abstract

Vitamins are essential to human health, and dietary supplements containing vitamins are widely used by individuals hoping to ensure they have adequate intake of these important nutrients. Measurement of vitamins in nutritional formulations is necessary to monitor regulatory compliance and in studies examining the nutrient intake of specific populations. Liquid chromatographic methods, primarily with UV absorbance detection, are well established for both fat- and water-soluble measurements, but they do have limitations for certain analytes and may suffer from a lack of specificity in complex matrices. Liquid chromatography-mass spectrometry (LC-MS) provides both sensitivity and specificity for the determination of vitamins in these matrices, and simultaneous analysis of multiple vitamins in a single analysis is often possible. In this work, LC-MS methods were developed for both fat- and water-soluble vitamins and applied to the measurement of these analytes in two NIST Standard Reference Materials. When possible, stable isotope labeled internal standards were employed for quantification.

Citation

Analytical Chemistry

Volume

83

Pub Type

Journals

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DOI Link

Keywords

dietary supplement, isotope dilution, liquid chromatography, mass spectrometry, vitamin

Citation

Phinney, K. , Rimmer, C. , Brown, J. , Sander, L. , Sharpless, K. and Wise, S. (2011), Isotope Dilution Liquid Chromatography - Mass Spectrometry Methods for Fat- and Water-Soluble Vitamins in Nutritional Formulations, Analytical Chemistry, [online], https://doi.org/10.1021/ac402689t (Accessed December 12, 2024)