A dielectrophoresis (DEP) microfluidic device that allows instantaneous trapping of cells for rapid return to cell growth conditions (within 5 minutes) and long-term cell experiments (cell proliferation, induction and differentiation) of embryonic P19 cells is presented. A hybrid cell adhesive material (HCAM) comprised of a positively charged polyelectrolyte, polyallylamine hydrochloride (PAH), and fibronectin (FN), is deposited onto DEP electrodes to serve as an adhesive material for the DEP-trapped cells. Cells adhere onto the HCAM and under flow conditions, do not detach from the surface when DEP forces are removed.
Proceedings Title: Proceedings of Micro Total Analysis Systems 2010, Chemical and Biological Microsystems Society
Conference Dates: October 3-7, 2010
Conference Location: Groningen, -1
Conference Title: The 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Pub Type: Conferences
dielectrophoresis, hybrid cell adhesive material, cell trapping, long-term cell assays