Epoxy functionalized supports are known to be suitable for industrial-scale immobilization of enzymes for biocatalysis. However, chemistry across these epoxy groups is known to affect the catalytic performance of the enzymes.In order to achieve a balance between immobilized enzyme activity and minimize enzyme leaching, immobilization supports with different physico-chemical properties were systematically studied. In the present study, we report immobilization of CAL B onto epoxy functionalized supports. CAL B was immobilized covalently on epoxy functionalized supports with different physico- chemical properties. Amongst them, Amberzyme and silanized silica support were selected as potential candidates and were studied in detail. In parallel, a microreactor based approach was developed in order to more systematically quantify the behavior of solid-supported catalysts in a continuous flow environment. Results indicates that covalently immobilized CAL B on silanized silica support showed better performance than that observed for methacrylate support, but lower biocatalytic performance as compared to physically immobilized CAL B on PMMA support.
Proceedings Title: American Chemical Society Spring 2010 National Meeting
Conference Dates: March 21-25, 2010
Conference Location: San Francisco, CA
Pub Type: Conferences
Microfluidics, CAL B, immobilization