Contact-less mapping of temperature distributions based on ratios of fluorescent signals is a ubiquitous temperature detection technique. However, application of calibration equations that relate fluorescence intensity ratios to temperature pose several practical challenges such as compensating for differences in reference temperature. In this report, we develop generalized calibration equations that can be applied for any value of reference temperature.
Citation: Analytical Chemistry
Pub Type: Journals
Rhodamine B, microfluidic channel, temperature, fluorescence