Focal Adhesions of Osteoblasts on Poly(d,l-lactide)/Poly(vinyl alcohol) Blends by Confocal Fluorescence Microscopy

Published

January 1, 2004

Author(s)

C A. Khatri, G Du, E S. Wu, Francis W. Wang

Abstract

Blends of poly(lactic acid) [PLA] and poly(vinyl alcohol) [PVOH] have been considered as biomaterials for tissue engineering scaffolds and for encapsulation of drugs. However, to our knowledge, there has been no attempt to use a combinatorial approach to characterize the biocompatibility of PLA/PVOH blends. Therefore, the purpose of the project described here was to measure focal adhesions, cell spread area, and cell number as indicators of biocompatibility for PLA/PVOH blends of various compositions. It was found that while the cells seeded on a PVOH film and on a PLA film exhibited prominent focal adhesions, the cell seeded on a blend film (70 % mass fraction of PLA) exhibited few focal adhesions.Similarly, the cell spread area and the cell number for the cells that were seeded on the blend films of intermediate compositions [mass fraction of (55, 70, and 80) % PLA] were less than those for the cells seeded on the PLA or PVOH films. The mechanisms of the diminished focal adhesions at these intermediate compositions are under investigation

Citation

World Biomaterials Congress

Volume

65(5)

Pub Type

Journals

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Keywords

focal adhesion, poly(d.l-lactide), poly(vinyl alcohol), polymer blends

Citation

Khatri, C. , Du, G. , Wu, E. and Wang, F. (2004), Focal Adhesions of Osteoblasts on Poly(d,l-lactide)/Poly(vinyl alcohol) Blends by Confocal Fluorescence Microscopy, World Biomaterials Congress, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=852258 (Accessed April 26, 2024)