Focal Adhesions of Osteoblasts on Poly(d,l-lactide)/Poly(vinyl alcohol) Blends by Confocal Fluorescence Microscopy
C A. Khatri, G Du, E S. Wu, Francis W. Wang
Blends of poly(lactic acid) [PLA] and poly(vinyl alcohol) [PVOH] have been considered as biomaterials for tissue engineering scaffolds and for encapsulation of drugs. However, to our knowledge, there has been no attempt to use a combinatorial approach to characterize the biocompatibility of PLA/PVOH blends. Therefore, the purpose of the project described here was to measure focal adhesions, cell spread area, and cell number as indicators of biocompatibility for PLA/PVOH blends of various compositions. It was found that while the cells seeded on a PVOH film and on a PLA film exhibited prominent focal adhesions, the cell seeded on a blend film (70 % mass fraction of PLA) exhibited few focal adhesions.Similarly, the cell spread area and the cell number for the cells that were seeded on the blend films of intermediate compositions [mass fraction of (55, 70, and 80) % PLA] were less than those for the cells seeded on the PLA or PVOH films. The mechanisms of the diminished focal adhesions at these intermediate compositions are under investigation
, Du, G.
, Wu, E.
and Wang, F.
Focal Adhesions of Osteoblasts on Poly(d,l-lactide)/Poly(vinyl alcohol) Blends by Confocal Fluorescence Microscopy, World Biomaterials Congress, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=852258
(Accessed June 10, 2023)