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Final Report for CCQM-K6.2: Determination of Total Cholesterol in Human Serum



Stephen Wise, Karen W. Phinney, David L. Duewer


Cholesterol is one of the most frequently measured substances in human blood/serum to assist in assessing the health status of individuals. Because of its clinical significance, CCQM-K6 Determination of Cholesterol in Serum was completed in 2000 as one of the first Key Comparison studies performed within the Organic Analysis Working Group (OAWG). The first Subsequent Key Comparison for cholesterol, CCQM-K6.1, was completed in 2001. Measurements for this second Subsequent, CCQM-K6.2, were completed in 2012. These Subsequent comparisons were conducted to enable CCQM members that had not participated in earlier studies to demonstrate their capabilities to measure a nonpolar (pKow < -2), low molecular mass (100 g/mol to 500 g/mol) metabolite in human serum at relatively high concentrations (1 mg/g to 3 mg/g) found in normal populations. Successful participation in CCQM-K6.2 demonstrated capabilities in analysis of complex biological matrices including sample preparation (extraction, derivatization), LC or GC separation, and quantification using an isotope dilution mass spectrometry approach. Given the excellent agreement between the anchor institute's results and robust consensus summary of the participants' values, the Key Comparison Reference Value (KCRV) for this study is the anchor institute's result. Seven of the nine participants demonstrated agreement with the KCRV.


CCQM-K6.2, Cholesterol, Subsequent Key Comparison


Wise, S. , Phinney, K. and Duewer, D. (2018), Final Report for CCQM-K6.2: Determination of Total Cholesterol in Human Serum, TBD, [online],, TBD (Accessed April 23, 2024)
Created June 6, 2018, Updated October 12, 2021