Many methods exist in the literature to modify surfaces with extracellular matrix (ECM) proteins prior to cell attachment. However, there are few studies that systematically compare surface characterization and cell response results among different modification methods. In this work, we compare results from physically adsorbed, chemically attached, and covalently bonded fibronectin and laminin to determine the best method to modify the deformable polydimethylsiloxane elastomer surface. Metrics to evaluate modification methods include amount, uniformity, hydrophilicity and roughness of ECM protein. Cell adhesion, proliferation, morphology and phenotype of vascular smooth muscle were also compared among the different methods.It was found that chemical attachment methods had the highest amount and uniformity of ECM protein over physically adsorbed and chemically bonded, although physical properties among the chemical methods could be very different. All chemical attachment methods gave similar results for cell adhesion, proliferation, area, aspect ratio and phenotype and were superior to physical adsorption. Cell adhesion and proliferation from covalent bonding methods were inferior to chemical attachment.
Citation: Journal of Biomedical Materials Research Part A
Pub Type: Journals
biomaterials, cellular response, protein adsorption, surface modification