Effects of Composite Filler Content and Surface Properties on Cellular Response

Published

August 16, 2009

Author(s)

Nancy J. Lin, Sheng Lin-Gibson

Abstract

Dimethacrylate-based composites have seen increased application in orthopedics, where materials are typically placed in direct contact with viable bone tissue. Since a number of material properties, including degree of conversion (DC), surface hydrophobicity, and surface topography, have been shown to affect cell growth on materials, a direct contact assay to evaluate a large parameter space would be useful. The objective of this study was to evaluate the response of osteoblast cells to dimethacrylate-based composites using a combinatorial approach. Substrates were prepared with five discrete variations in filler content/type, each containing a continuous gradient in irradiation intensity, resulting in variations in DC, surface topography, and water contact angle. As DC decreased, roughness and hydrophobicity increased, cell density decreased for most compositions, and cell area decreased. Cell viability was high (> 80 %) for all locations. Overall, a combination of interrelated material properties, and not just DC, influenced the cell response. A single property did not dominate the cell response, thus the combinatorial approach allowed for simultaneous variations in multiple properties and may be useful in leading to a set of parameters that best optimizes the osteoblast response for a specific application. Support: NIST/NIDCR Interagency Agreement YI-DE-7005-01.

Proceedings Title

238th ACS National Meeting & Exposition, Fall 2009, Division of Polymer Materials: Science & Engineering

Conference Dates

August 16-20, 2009

Conference Location

Washington, DC

Conference Title

238th ACS National Meeting & Exposition, Fall 2009

Pub Type

Conferences

Keywords

combinatorial, composite, contact angle, cytotoxicity, degree of conversion, gradient, osteoblast, photopolymerization, surface topography

Citation

Lin, N. and Lin-Gibson, S. (2009), Effects of Composite Filler Content and Surface Properties on Cellular Response, 238th ACS National Meeting & Exposition, Fall 2009, Division of Polymer Materials: Science & Engineering , Washington, DC (Accessed April 19, 2024)