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Development of Botanical and Marine Oil Standard Reference Materials for Fatty Acids



Michele M. Schantz, Lane C. Sander, Katherine E. Sharpless, Stephen A. Wise, James H. Yen, A. NguyenPho, Joseph M. Betz


As part of a collaboration with the National Institutes of Health’s Office of Dietary Supplements and the Food and Drug Administration’s Center for Drug Evaluation and Research, the National Institute of Standards and Technology has developed Standard Reference Material (SRM) 3274 Botanical Oils Containing Ω-3 and Ω-6 Fatty Acids and SRM 3275 Ω-3 and Ω-6 Fatty Acids in Fish Oil. SRM 3274 consists of one ampoule of each of four seed oils (3274-1 Borage (Borago officinalis), 3274-2 Evening Primrose (Oenothera biennis), 3274-3 Flax (Linium usitatissimum), and 3274-4 Perilla (Perilla frutescens), and SRM 3275 consists of two ampoules of each of three fish oils (3275-1 a concentrate high in docosahexaenoic acid, 3275-2 an anchovy oil high in docosahexaenoic acid and eicosapentaenoic acid, and 3275-3 a concentrate containing 60% long-chain omega-3 fatty acids. Each oil has certified and reference mass fraction values for up to 20 fatty acids. The fatty acid mass fraction values are based on results from analyses using gas chromatography with flame ionization detection (GC-FID) and mass spectrometry (GC/MS). These SRMs will complement other reference materials currently available with mass fractions for similar analytes and are part of a series of SRMs being developed for dietary supplements.
Analytical and Bioanalytical Chemistry


omega-3 fatty acids, omega-6 fatty acids, plant oil, fish oil, reference material


Schantz, M. , Sander, L. , Sharpless, K. , Wise, S. , Yen, J. , NguyenPho, A. and Betz, J. (2013), Development of Botanical and Marine Oil Standard Reference Materials for Fatty Acids, Analytical and Bioanalytical Chemistry, [online], (Accessed July 15, 2024)


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Created May 15, 2013, Updated November 10, 2018