Hayes, H.
, Wilson, W.
, Sander, L.
, Wise, S.
and Campiglia, A.
(2018),
Determination of polycyclic aromatic hydrocarbons with molecular mass 302 in standard reference material 1597a by reversed-phase liquid chromatography and stop-flow fluorescence detection, Analytical Methods, [online], https://doi.org/10.1039/c8ay00760h, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=924986
(Accessed December 8, 2024)
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Determination of polycyclic aromatic hydrocarbons with molecular mass 302 in standard reference material 1597a by reversed-phase liquid chromatography and stop-flow fluorescence detection
Published
Author(s)
Hugh Hayes, , , , Andres Campiglia
Abstract
The identification of isomeric polycyclic aromatic hydrocarbons (PAHs) in complex samples via reversed-phase liquid chromatography (RPLC) with fluorescence detection (FL) is normally based on matching the chromatographic retention times of suspected peaks of interest with reference standards. Since no spectral information is obtained during the chromatographic run, the accurate identification of co-eluting PAHs with similar chromatographic behaviors requires confirmation with additional chromatographic methods. This is particularly true for the analysis of PAH isomers with the molecular mass (MM, g/mol) 302. The work presented here explores the information content of room-temperature fluorescence spectra for the analysis of PAHs with MM 302 in the Standard Reference Material (SRM) 1597a. Fluorescence spectra were recorded under stop-flow conditions with the aid of commercial HPLC system. Of the 21 MM 302 PAHs known to be present in the SRM 1597a, 20 were tentatively identified based on retention times and the presence of 18 was confirmed on the basis of excitation and emission spectral profiles.Citation
Analytical Methods
Volume
10
Issue
23
Pub Type
Journals
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Keywords
reversed-phase liquid chromatography, polycyclic aromatic hydrocarbons, fluorescence, normal- phase liquid chromatography
Citation
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Created June 20, 2018, Updated October 12, 2021