Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Determination of Organic Acids in Vaccinium Berry Standard Reference Materials



Melissa M. Phillips, Lane C. Sander, Katherine E. Sharpless, Stephen A. Wise, Catherine A. Rimmer, Ryan Case, James H. Yen


Nine organic acids (citric acid, galacturonic acid, glycolic acid, isocitric acid, malic acid, oxalic acid, quinic acid, shikimic acid, and tartaric acid) and two anions (phosphate and sulfate) were determined in a suite of Vaccinium berry containing dietary supplement Standard Reference Materials (SRMs). Following solvent extraction, three independent methods were utilized in the quantification of these compounds. The first method involved reversed phase liquid chromatography with ultraviolet absorbance detection at 210 nm and isotope dilution mass spectrometry. The second method utilized ion chromatography with conductivity detection. Finally, gas chromatography with isotope dilution mass spectrometry detection was used following derivatization with N-methyl-N-trifluoroacetamide (MSTFA). The combined data from all three methods was used for the assignment of organic acid levels in the seven candidate SRMs.
Analytical and Bioanalytical Chemistry


Organic acids, berry, liquid chromatography, gas chromatography, ion chromatography, mass spectrometry, isotope dilution


Phillips, M. , Sander, L. , Sharpless, K. , Wise, S. , Rimmer, C. , Case, R. and Yen, J. (2010), Determination of Organic Acids in Vaccinium Berry Standard Reference Materials, Analytical and Bioanalytical Chemistry (Accessed July 21, 2024)


If you have any questions about this publication or are having problems accessing it, please contact

Created June 27, 2010, Updated February 19, 2017