Determination of 19-Norandrosterone in CCQM-P68. NIST Results Using an Isotope Dilution Liquid Chromatography/Tandem Mass Spectrometry Method

Published

October 16, 2008

Author(s)

Susan Tai, Bei Xu, Michael J. Welch

Abstract

The U.S. National Institute of Standards and Technology (NIST) participated in an international interlaboratory study under the auspices of the Consultative Committee for Amount of Substance (CCQM) for the determination of 19-norandrosterone (19-NA) in urine, the principal metabolite of nandrolone and certain other synthetic testosterone substitutes banned for use by the World Antidoping Agency (WADA). Prior to this study, NIST developed a candidate reference measurement procedure based upon isotope dilution liquid chromatography/mass spectrometry. This method was applied to a urine sample distributed to the participants in the study by the Australian National Measurement Institute, Pymble, Australia. The NIST results were in very good agreement with those from the other participants, all of whom used methods based upon gas chromatography/mass spectrometry. All known significant sources of uncertainty were evaluated, resulting in a relative expanded uncertainty of less than 5%.

Citation

Accreditation and Quality Assurance

Pub Type

Journals

Keywords

19-NA, 19-norandrosterone, CCQM, isotope dilution, liquid chromatography/tandem mass spe, reference measurement procedure

Citation

Tai, S. , Xu, B. and Welch, M. (2008), Determination of 19-Norandrosterone in CCQM-P68. NIST Results Using an Isotope Dilution Liquid Chromatography/Tandem Mass Spectrometry Method, Accreditation and Quality Assurance (Accessed December 6, 2024)