Controlled Release of Transforming Growth Factor From Composite Bone Grafts Consisting of Calcium Phosphate Cement and Biodegradable Particulates

Published

January 1, 2003

Author(s)

C A. Khatri, Francis W. Wang

Abstract

Calcium phosphate cement (CPC) was improved by incorporating porogens to induce macropores and trasforming growth factor to stimulate bone growth. A paste was made from 0.0577 g of salicylic acid crystals (0.4 volume fraction) and 0.1923 g of CPC (0.6 volume fraction), and 0.062 g of 4 mM hydrochloric acid (HCl) containing bovine serum albumin (BSA, mass fraction of 1 %). The liquid component also contained 200 ng of TGF-b1 reconstituted in 4 mM HCl. Similarly, a paste consisting of CPC and mannitol was similarly prepared, using 0.1905 gm of CPC powder (0.6 volume fraction), 0.0595 gm of mannitol crystals (0.4 volume fraction), and 100 ng of TGF-b1 in the liquid component. The ELISA analysis of the aliquots collected at time intervals yielded the amount of TGF-b1 released as a function of time showing that the release of proteins from composite grafts can be modulated by the dissolution rate of porogens.

Citation

Society for Biomaterials Meeting

Volume

19(8)

Pub Type

Journals

Download Paper

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Keywords

bonegrafts, calcium phosphate cements, controlled release, transforming growth factor

Citation

Khatri, C. and Wang, F. (2003), Controlled Release of Transforming Growth Factor From Composite Bone Grafts Consisting of Calcium Phosphate Cement and Biodegradable Particulates, Society for Biomaterials Meeting, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=852102 (Accessed December 3, 2024)