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Construction of a Dual Protease Column, Subzero (-30 °C) Chromatography System and Multi-channel Precision Temperature Controller for Hydrogen-Deuterium Exchange Mass Spectrometry



Jeffrey W. Hudgens


This tutorial provides mechanical drawings, electrical schematics, parts lists, stereolithography (STL) files for producing three-dimensional (3D)-printed parts, initial graphics exchange specification (IGS) files for automated machining, and instructions necessary for construction of a dual protease column, subzero, liquid chromatography system for hydrogen- deuterium exchange mass spectrometry (HDX-MS). Electro-mechanical schematics for construction of two multi-zone temperature controllers that regulate to +/-0.05 °C are also included in this tutorial.
Journal of Research (NIST JRES) -


chromatography, hydrogen-deuterium exchange, mass spectrometry, precision, peptide, protein, proteolysis, proteomics, reference material, temperature control.
Created August 11, 2020