Hudgens, J.
(2020),
Construction of a Dual Protease Column, Subzero (-30 °C) Chromatography System and Multi-channel Precision Temperature Controller for Hydrogen-Deuterium Exchange Mass Spectrometry, Journal of Research (NIST JRES), National Institute of Standards and Technology, Gaithersburg, MD, [online], https://doi.org/10.6028/jres.125.025
(Accessed October 11, 2025)
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Construction of a Dual Protease Column, Subzero (-30 °C) Chromatography System and Multi-channel Precision Temperature Controller for Hydrogen-Deuterium Exchange Mass Spectrometry
Published
Author(s)
Abstract
This tutorial provides mechanical drawings, electrical schematics, parts lists, stereolithography (STL) files for producing three-dimensional (3D)-printed parts, initial graphics exchange specification (IGS) files for automated machining, and instructions necessary for construction of a dual protease column, subzero, liquid chromatography system for hydrogen- deuterium exchange mass spectrometry (HDX-MS). Electro-mechanical schematics for construction of two multi-zone temperature controllers that regulate to +/-0.05 °C are also included in this tutorial.Citation
Journal of Research (NIST JRES) -
Volume
125
NIST Pub Series
Pub Type
NIST Pubs
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Keywords
chromatography, hydrogen-deuterium exchange, mass spectrometry, precision, peptide, protein, proteolysis, proteomics, reference material, temperature control.
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Created August 11, 2020, Updated May 4, 2021