Hudgens, J.
(2020),
Construction of a Dual Protease Column, Subzero (-30 °C) Chromatography System and Multi-channel Precision Temperature Controller for Hydrogen-Deuterium Exchange Mass Spectrometry, Journal of Research (NIST JRES), National Institute of Standards and Technology, Gaithersburg, MD, [online], https://doi.org/10.6028/jres.125.025
(Accessed October 27, 2025)
Official websites use .gov
A .gov website belongs to an official government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.
Construction of a Dual Protease Column, Subzero (-30 °C) Chromatography System and Multi-channel Precision Temperature Controller for Hydrogen-Deuterium Exchange Mass Spectrometry
Published
Author(s)
Abstract
This tutorial provides mechanical drawings, electrical schematics, parts lists, stereolithography (STL) files for producing three-dimensional (3D)-printed parts, initial graphics exchange specification (IGS) files for automated machining, and instructions necessary for construction of a dual protease column, subzero, liquid chromatography system for hydrogen- deuterium exchange mass spectrometry (HDX-MS). Electro-mechanical schematics for construction of two multi-zone temperature controllers that regulate to +/-0.05 °C are also included in this tutorial.Citation
Journal of Research (NIST JRES) -
Volume
125
NIST Pub Series
Pub Type
NIST Pubs
Download Paper
Keywords
chromatography, hydrogen-deuterium exchange, mass spectrometry, precision, peptide, protein, proteolysis, proteomics, reference material, temperature control.
Citation
Issues
If you have any questions about this publication or are having problems accessing it, please contact [email protected].
Created August 11, 2020, Updated May 4, 2021