Concentration Dependent Neural Differentiation and Neurite Extension from mouse ESC on Primary Amine-derivatized Surfaces

Published

February 27, 2013

Author(s)

Laura A. Smith Callahan, Yanrui Ma, Christopher Stafford, Matthew L. Becker

Abstract

We demonstrate a versatile, well controlled, and highly reproducible surface density gradient fabrication strategy that allows for precise control over surface functionality and slope. Our approach involves of a novel "vacuum away" channel confinement vapor deposition method, which overcomes challenges with regard to chemical tunability concentration control and has potential for translation into complicated geometries that are present in the aforementioned gradient formation methods. ESC differentiation results illustrate how amine concentration can affect cellular function and provides new information on the optimal therapeutic range of amines on ESC neural differentiation while providing a novel platform to study the variations affect neural differentiation and warrant further investigation. Future studies will translate these findings to clinically relevant substrates.

Citation

Biomaterials Science

Volume

1

Issue

5

Pub Type

Journals

Keywords

monolayers, self-assembly, gradients, differentiation, cells, surfaces

Citation

Smith Callahan, L. , Ma, Y. , Stafford, C. and Becker, M. (2013), Concentration Dependent Neural Differentiation and Neurite Extension from mouse ESC on Primary Amine-derivatized Surfaces, Biomaterials Science (Accessed December 5, 2024)