Certification of Standard Reference Materials® 2969 and 2970: Vitamin D Metabolites in Frozen Human Serum (Total 25-Hydroxyvitamin D Low Level) and (25-Hydroxyvitamin D2 High Level)

Published

September 2, 2021

Author(s)

Grace Hahm, Michael Nelson, Johanna Camara, Blaza Toman

Abstract

Standard Reference Materials (SRMs) 2969 and 2970 are intended for 1) use in validating methods for determining concentrations of 25-hydroxyvitamin D2 (25(OH)D2) and 25-hydroxyvitamin D3 (25(OH)D3) in human serum and plasma and 2) value assigning in house produced control materials analyzed using those methods. A unit of SRM 2969 consists of two vials of frozen serum with a low total 25-hydroxyvitamin D (25(OH)D, the sum of 25(OH)D2 plus 25(OH)D3) concentrations. A unit of SRM 2970 consists of two vials of frozen serum with a relatively high 25(OH)D2 concentration. This publication documents the production, analytical methods, and statistical evaluations involved in characterization of these products.

Citation

Special Publication (NIST SP) - 260-210

Report Number

260-210

NIST Pub Series

Special Publication (NIST SP)

Pub Type

NIST Pubs

Download Paper

https://doi.org/10.6028/NIST.SP.260-210

Local Download

Keywords

Human serum, Standard Reference Material (SRM), 25-hydroxyvitamin D2, (25(OH)D2), 25-hydroxyvitamin D3, (25(OH)D3), total 25-hydroxyvitamin D, (25(OH)D)

Citation

Hahm, G. , Nelson, M. , Camara, J. and Toman, B. (2021), Certification of Standard Reference Materials® 2969 and 2970: Vitamin D Metabolites in Frozen Human Serum (Total 25-Hydroxyvitamin D Low Level) and (25-Hydroxyvitamin D2 High Level), Special Publication (NIST SP), National Institute of Standards and Technology, Gaithersburg, MD, [online], https://doi.org/10.6028/NIST.SP.260-210, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=932138 (Accessed October 9, 2025)