Zheng Zhang

Staff Employment

2015 – present, National Institute of Standards and Technology, Gaithersburg, MD

2014 – 2015, Poochon Scientific, Frederick, MD

2012 – 2014, Pediatric Endocrinology, NIH Clinical Center, Bethesda, MD

2005 - 2007, Department of Nephrology, Internal Medicine, VCU Medical Center, Richmond, VA

2000 - 2004, Lab of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, NIH, Bethesda, MD

Staff Education

2012, ECFMG Certificate from Educational Commission for Foreign Medical Graduates
1999 - 2000, Residency, Pediatrics&Genetics, Peking Union Medical College Hospital
1997 - 1999, Residency, Internal Medicine, Hunan Medical University
1999, M.S., Endocrinology, Internal Medicine, Hunan Medical University
1999, M.D., 7-year program in Clinical Medicine, Hunan Medical University

Awards

Certificate of recognition for outstanding services to Mobile Med and the Community, 2012.
Gave an invited tutorial seminar on precocious puberty at the Peds Endo Weekly Teaching Conference at NIH Clinical Center in August 2012.
Research work on the association of osmotic and oxidative stress was featured on the cover page with a commentary in PNAS USA, 101 (25), 2004.

Publications

An Improved Sample Preparation Method for Protein and Peptide Identification from Human Hair

November 27, 2023

Author(s)

Zheng Zhang, William E. Wallace, Guanghui Wang, Meghan Burke Harris, Yi Liu, Sergey Sheetlin, Stephen E. Stein

A fast and sensitive Direct Extraction (DE) method developed in our group can efficiently extract proteins in 30 min from a 5 cm-long hair strand. Previously we

Sensitive Method for the Confident Identification of Genetically Variant Peptides in Human Hair

October 31, 2019

Author(s)

Zheng Zhang, Meghan Burke, William E. Wallace, Yuxue Liang, Sergey L. Sheetlin, Yuri Mirokhin, Dmitrii Tchekhovskoi, Stephen Stein

Recent reports have demonstrated that genetically variant peptides (GVPs) derived from human hair shaft proteins can be used to differentiate individuals of

False Discovery Rate Estimation for Hybrid Mass Spectral Library Search Identifications in Bottom-up Proteomics

July 31, 2019

Author(s)

Meghan C. Burke, Zheng Zhang, Yuri A. Mirokhin, Dmitrii V. Tchekhovskoi, Yuxue Liang, Stephen E. Stein

We present a method for FDR estimation of mass spectral library search identifications made by a recently developed method for peptide identification, the

Reverse and Random Decoy Methods for False Discovery Rate Estimation in High Mass Accuracy Peptide Spectral Library Searches

February 2, 2018

Author(s)

Zheng Zhang, Meghan C. Burke, Yuri A. Mirokhin, Dmitrii V. Tchekhovskoi, Sanford P. Markey, Wen Yu, Raghothama Chaerkady, Sonja Hess, Stephen E. Stein

Spectral library searching (SLS) is an attractive alternative to sequence database searching (SDS) for peptide identification due to its speed, sensitivity, and

Interconversion of Peptide Mass Spectral Libraries Derivatized with iTRAQ or TMT Labels

July 7, 2016

Author(s)

Zheng Zhang, Xiaoyu Yang, Yuri A. Mirokhin, Dmitrii V. Tchekhovskoi, Weihua Ji, Sanford P. Markey, Pedatsur Neta, Bowen A. Michael, Stephen E. Stein

Derivatizing peptides with isobaric tags such as iTRAQ and TMT is widely employed in proteomics due to their ability to multiplex quantitative measurements. We

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Staff Employment

2015 – present, National Institute of Standards and Technology, Gaithersburg, MD

2014 – 2015, Poochon Scientific, Frederick, MD

2012 – 2014, Pediatric Endocrinology, NIH Clinical Center, Bethesda, MD

2005 - 2007, Department of Nephrology, Internal Medicine, VCU Medical Center, Richmond, VA

2000 - 2004, Lab of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, NIH, Bethesda, MD

Staff Education

2012, ECFMG Certificate from Educational Commission for Foreign Medical Graduates
1999 - 2000, Residency, Pediatrics&Genetics, Peking Union Medical College Hospital
1997 - 1999, Residency, Internal Medicine, Hunan Medical University
1999, M.S., Endocrinology, Internal Medicine, Hunan Medical University
1999, M.D., 7-year program in Clinical Medicine, Hunan Medical University

Zheng Zhang (Fed)

Research Chemist

Awards

Publications

An Improved Sample Preparation Method for Protein and Peptide Identification from Human Hair

Sensitive Method for the Confident Identification of Genetically Variant Peptides in Human Hair

False Discovery Rate Estimation for Hybrid Mass Spectral Library Search Identifications in Bottom-up Proteomics

Reverse and Random Decoy Methods for False Discovery Rate Estimation in High Mass Accuracy Peptide Spectral Library Searches

Interconversion of Peptide Mass Spectral Libraries Derivatized with iTRAQ or TMT Labels