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Raman Hlushko (Assoc)

Materials Science and Engineering, Research Associate IBBR

Experimental Skills and Interests

  • Organic synthesis: emulsion polymerization, RAFT, ATRP, and polymer modification
  • Polymer characterization: thermal analysis, DMA, NMR, FTIR, UV-Vis, rheology
  • Chromatography: HPLC, GC, SEC, IEC, TLC
  • Coating preparation: spin, dip, and spray coating deposition, layer-by-layer assembly
  • Surface characterization: optical microscopy, scanning electron microscopy, profilometry, ellipsometry, atomic force microscopy, contact angle measurements
  • High-resolution materials characterization: Cryogenic transmission electron microscopy, electron energy lost spectrometry, Raman confocal microscopy, nanoindentation, nanoDMA
  • Polymers and nanoparticle characterization: DLS, SLS, FCS
  • Neutron Reflectometry: sample preparation, experiment design, and data fitting

Recent Publications (Google Scholar)

  1. R Hlushko, A Marin, A Chowdhury, and AK Andrianov, Macromolecular dimensions of a synthetic polyelectrolyte as a factor in its interactions with protein and cells: desirability for longer chains, Journal of Materials Chemistry B 14 (2), 563-571, 2026. https://doi.org/10.1039/D5TB02163D
  2. R Hlushko, VM Prabhu, and AK Andrianov, Cyclic Macromolecular Chains Visualized by Cryo-EM and AFM Reveal a Ring Expansion Polymerization Mechanism in a Classical Synthetic Pathway to Polyphosphazenes, ACS Macro Letters 14 (10), 1476-1483, 2025. https://doi.org/10.1021/acsmacrolett.5c00529
  3. AK Andrianov, A Marin, S Jeong, L Kulakova, A Chowdhury, R Hlushko, A Das, F Moy, EA Toth, RK Ernst, and TR Fuerst, Polyphosphazene-Mediated Assembly of TLR4 and TLR7/8 Agonists Enables a Potent Nano-Adjuvant Delivery System for Hepatitis C Virus Vaccine Antigens, Vaccines 13 (10), 1012, 2025. https://doi.org/10.3390/vaccines13101012
  4. TR Fuerst, A Marin, S Jeong, L Kulakova, R Hlushko, K Gorga, EA Toth, NJ Singh, and AK Andrianov, Virus-Mimicking Polymer Nanocomplexes Co-Assembling HCV E1E2 and Core Proteins with TLR 7/8 Agonist—Synthesis, Characterization, and In Vivo Activity, Journal of Functional Biomaterials 16 (1), 34, 2025. https://doi.org/10.3390/jfb16010034
  5. R Hlushko, A Marin, and AK Andrianov, Protein–polyelectrolyte complexation: effects of sterically repulsive groups, macromolecular architecture and hierarchical assembly, Soft Matter 21, 418-426, 2025. https://doi.org/10.1039/D4SM01254B
  6. HD Tagad, A Marin, R Hlushko, and AK Andrianov, Hydrolytically Degradable Zwitterionic Polyphosphazene Containing HEPES Moieties as Side Groups, Biomacromolecules 25, 6791-6800, 2024. https://doi.org/10.1021/acs.biomac.4c01008
  7. VH Leyva-Grado, A Marin, R Hlushko, AS Yunus, D Promeneur, A Luckay, GG. Lazaro, S Hamm, AS Dimitrov, CC Broder, and AK Andrianov, Nano-assembled polyphosphazene delivery system enables effective intranasal immunization with Nipah virus subunit vaccine, , ACS Applied Bio Materials 7, 4133-4141, 2024. https://doi.org/10.1021/acsabm.4c00441
  8. R Hlushko, E Pozharski, VM Prabhu, AK Andrianov, Directly visualizing individual polyorganophosphazenes and their single-chain complexes with proteins, Communications Materials 5, 36, 2024. https://doi.org/10.1038/s43246-024-00476-6

  9. J Brito, J Moon, R Hlushko, A Aliakseyeu, AK Andrianov, and SA Sukhishvili, Engineering Degradation Rate of Polyphosphazene-Based Layer-by-Layer Polymer Coatings, Journal of Functional Biomaterials 15, 26, 2024. https://doi.org/10.3390/jfb15020026


     

Selected Publications

Created January 27, 2024, Updated February 2, 2026
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