Skip to main content
U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.


Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

Jeeseong C. Hwang

Jeeseong Hwang is a research biophysicist in the Physical Measurement Laboratory at the National Institute of Standards and Technology (NIST). His work at NIST began in 1994 with an award for a National Research Council Postdoctoral Fellowship to study near-field optical scanning probe microscopy of nanoscale biomimetic materials and structures. Before coming to NIST, he was a research scientist in an immunology laboratory at the biology department of the Johns Hopkins University, investigating biophysical aspects of immune response of human cells using super-resolution near-field scanning optical microscopy and other laser-based optical imaging techniques in collaboration with AT&T Bell Laboratories. His Ph. D thesis work at the physics department of Michigan State University was on scanning probe microscopy of nanoscale quantum structures and metal surfaces.

His research in the Molecular and BioPhotonics Project focuses on optics and nanobiophotonics for optical medical imaging and quantitative biophysics, involving development and application of the following areas: (1) multimodal molecular imaging techniques; (2) integrated optical analysis platforms for multiplexed bio-assays; (3) bio-inspired assembly of nanoscale building blocks for optical bio-sensors; and (4) quantitative image analysis software for multiplexed dynamic bio-assays.

Recent contributions to professional societies include serving on a steering committee of the IEEE-Nanotechnology Conference in 2010, a program committee of the annual SPIE BiOS convention since 2006, and a ISO scanning probe microscopy subcommittee in 2005-2007. He received a NIST/DoC Silver Medal for "the development of an innovative nanotechnology tool for the highly sensitive and selective optical detections of bacterial pathogens" in 2008. He has authored 53 technical papers, delivered 28 invited talks, and holds two U.S. Patents.

Research Projects:

  • Advanced integrated microscopy
    • Time-correlated confocal nano-spectroscopic microscopy of single and clustered nanoprobes
    • Integrated microscopy of photo-excited nanoparticles and related processes
    • Optical tweezers and single molecule microscopy of biomolecular interactions
    • Multimodal optical microscopy of molecular dynamics and cellular processes
    • Hyperspectral microscopy of in vivo simulated cell culture
  • Image analysis software for quantitative and dynamic molecular imaging
    • NIST's Integrated Colony Enumerator (NICE)
    • Single particle tracking analysis
    • Dynamic imaging of cellular processes
  • Next generation optical bioassays
    • Nano-biosensors of nanoscale building blocks
    • Multiplexed high-throughput biomolecular assays
    • Fusion proteins for dynamical studies of infectious diseases


Dual-comb photoacoustic spectroscopy

Jacob T. Friedlein, Esther Baumann, Kimberly Briggman, Gabriel M. Colacion, Fabrizio R. Giorgetta, Daniel I. Herman, Nathan R. Newbury, Jeeseong Hwang, Ian R. Coddington, Kevin C. Cossel, Gabriel Ycas, Christopher Yung, Eli V. Hoenig, Edgar F. Perez, Aaron Goldfain
Spectrally-resolved photoacoustic imaging is a promising technique for label-free imaging in optically scattering materials. However, this technique often


Optical Phantom for Producing a Time-Resolved Diffuse Reflectance Spectrum

NIST Inventors
Jeeseong C. Hwang
Patent Description Physikalisch-Technische Bundesanstalt (PTB) and NIST jointly developed a concept for a waveform synthesizer which can generate a controlled waveform with several picosecond (ps) time resolution. In a light-diffusing material, characteristics of the time-of-flight distribution (e.g
Created October 9, 2019