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Jeeseong C. Hwang (Fed)

Jeeseong Hwang is a research biophysicist in the Applied Physics Division in the Physical Measurement Laboratory at the National Institute of Standards and Technology (NIST). His work at NIST began in 1994 with an award for a National Research Council Postdoctoral Fellowship to study near-field optical scanning probe microscopy of nanoscale biomimetic materials and structures. Before coming to NIST, he was a research scientist in an immunology laboratory at the biology department of the Johns Hopkins University, investigating biophysical aspects of immune response of human cells using super-resolution near-field scanning optical microscopy and other laser-based optical imaging techniques in collaboration with AT&T Bell Laboratories. His Ph.D. thesis work at the physics department of Michigan State University was on scanning probe microscopy of nanoscale quantum structures and metal surfaces.

His research in the Molecular and BioPhotonics Project focuses on optics and nanobiophotonics for optical medical imaging and quantitative biophysics, involving development and application of the following areas: (1) multimodal molecular imaging techniques; (2) the development of phantoms for optical medical imaging; (3) bio-inspired assembly of nanoscale building blocks for optical bio-sensors; and (4) quantitative image analysis software.

Recent contributions to professional societies include serving on the committee for the 'Quality and Reliability of Biomedical Devices' program at annual SPIE BiOS Conference since 2006. He received a NIST/Department of Commerce Silver Medal for "the development of an innovative nanotechnology tool for the highly sensitive and selective optical detections of bacterial pathogens" in 2008. He has authored 90 technical papers, delivered 57 invited talks, and holds six U.S. Patents.

Research Projects

  • Advanced integrated microscopy
    • Time-correlated confocal nano-spectroscopic microscopy of single and clustered nanoprobes
    • Multimodal optical microscopy of molecular dynamics and cellular processes
    • Hyperspectral microscopy of in vivo simulated cell culture
  • Image analysis software for quantitative imaging
    • NIST's Integrated Colony Enumerator (NICE)
    • Single particle tracking analysis
    • Hyperspectral Imaging for Tumor Margin Analysis
  • Optical Medical Imaging
    • Optical Coherence Tomography
    • Photoacoustic Tomography and Microscopy
    • Phantom Development

Selected Publications


Spatial frequency domain Mueller matrix imaging

Joseph Chue-Sang, Aaron Goldfain, Jeeseong C. Hwang, Thomas A. Germer
We combine Mueller matrix polarimetry (MMP) with spatial frequency domain imaging (SFDI) to create a technique that is sensitive to near-surface material

Patents (2018-Present)

Photoacoustic Photon Meter And Process For Measuring Photon Fluence

NIST Inventors
Kimberly A. Briggman , Chris Yung , Jeeseong C. Hwang and John H. Lehman
A photoacoustic photon meter includes: a photoacoustic generative array including carbon nanotubes disposed in a photoacoustic generating pattern, such that the carbon nanotubes: receive photons comprising optical energy, and produce thermal energy from the optical energy; and a superstratum

Optical Phantom for Producing a Time-Resolved Diffuse Reflectance Spectrum

NIST Inventors
Jeeseong C. Hwang
An optical phantom produces a time-resolved diffuse reflectance spectrum and includes: a light source; a spatial light modulator; and an optical delay line including optical fibers of different length that produce different time-of-flight distributions, such that different time-of-flight
Created October 9, 2019, Updated May 9, 2023