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Edward Kwee

Research Biologist/NRC Postdoctoral Research Associate

Edward Kwee is a NIST-NRC Postdoctoral Research Associate working with his advisor, John Elliott, in the Cell Systems Science Group. He completed his PhD in Biomedical Engineering from Case Western Reserve University in 2016. His dissertation research was performed in the lab of Dr. George Muschler at the Cleveland Clinic where Edward worked on characterizing bone derived stem and progenitor cell heterogeneity using time lapse imaging, image analysis, and cell picking.  The goal of his current work is to contribute to traceable, non-invasive metrology in cell therapy biomanufacturing.

Research Interests

Induced pluripotent stem cells (iPSCs) are reprogrammed cells with varying biological potential for use in cell therapy products. There is a need for objective, non-invasive live cell methods to identify differences in pluripotency and aid in the manufacturing of desired iPSCs. Edward’s research focuses on the use of quantitative phase imaging (QPI) as a traceable, quantitative measure of iPSC potency. QPI provides quantitative measurements of the change in the phase of light through cells and their surroundings. This enables measurement of cell thickness, volume, and mass. Edward is also working on the development of traceable QPI reference materials to enable reproducible and comparable QPI measurements between different experiments and laboratories.

 

Quantitative phase image of induced pluripotent stem cells and reference beads
Quantitative Phase Imaging. Left) Induced Pluripotent Stem Cell Colony: Live QPI image of induced pluripotent stem cell colony. Right) QPI image of candidate reference beads. Image intensity is proportional to optical pathlength difference in nanometers.

 

Publications

  1. Kwee, E., et al. “Integrated Colony Imaging, Analysis, and Selection Device for Regenerative Medicine.” Journal of Laboratory Automation, 2016.
  2. Powell, K., Kwee, E., et al.. "Variability in subjective review of umbilical cord blood colony forming unit assay." Cytometry Part B: Clinical Cytometry, 2016. 90(6): 517-524.
  3. Kwee, E., Powell, K., and Muschler, G. Characterization of connective tissue progenitors through phase contrast and multicolor fluorescence time-lapse microscopy ", Proc. SPIE 9328, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII, 93280O, 2015.

Awards

National Research Council Postdoctoral Research Associateship, 2016

AAAS/Science Program for Excellence in Science, 2015

Alpha Epsilon: Honor Society of Biological Engineering, 2010

Cornell Engineering Co-op of the Year, 2009

Publications

Created December 10, 2018, Updated November 14, 2019