DNA damage products (5'R)- and (5'S)-8,5'-cyclo-2'-deoxyadenosines as potential biomarkers for atherosclerosis in human urine

Published

February 23, 2012

Author(s)

M Miral Dizdar, Pawel Jaruga, R Olinski, Rafal Rozalski

Abstract

Rationale: We hypothesized that oxidatively induced DNA damage products (5'R)-8,5'-cyclo-2'-deoxyadenosine (R-cdA) and (5'S)-8,5'-cyclo-2'-deoxyadenosine (S-cdA) may be well-suited as biomarkers for atherosclerosis in human urine. Objective: We tested this hypothesis by accurately measuring the levels of R-cdA and S-cdA in urines of atherosclerosis and healthy individuals. Methods and Results: We collected urine samples from 22 patients with clinical atherosclerosis and from 22 healthy individuals. The levels of R-cdA and S-cdA, and another typical product of DNA damage 8-hydroxy-2'-deoxyguanosine (8-OH-dG) were simultaneously measured using liquid chromatography-tandem mass spectrometry with isotope dilution. We show the presence of R-cdA and S-cdA at significantly greater concentrations in urine of atherosclerosis patients than in that of healthy individuals with a p-value

Citation

ACS Biochemistry

Issue

51

Pub Type

Journals

Download Paper

https://doi.org/10.1021/bi201912c

Keywords

atherosclerosis, biomarkers, 8, 5'-cyclo-2'-deoxyadenosines, DNA damage, oxidative stress

Citation

, M. , Jaruga, P. , Olinski, R. and , R. (2012), DNA damage products (5'R)- and (5'S)-8,5'-cyclo-2'-deoxyadenosines as potential biomarkers for atherosclerosis in human urine, ACS Biochemistry, [online], https://doi.org/10.1021/bi201912c (Accessed October 11, 2025)