Certification of Standard Reference Material® 3666: Albumin and Creatinine in Frozen Human Urine - INCLUDES UPDATES AS OF 07-02-2025

Published

September 2, 2025

Author(s)

Ashley Green, Johanna Camara, Nathanael Heckert

Abstract

The National Institute of Standards and Technology (NIST) Standard Reference Material® (SRM®) 3666 Albumin and Creatinine in Frozen Human Urine is a secondary measurement standard for albumin and creatinine in human urine. The material is intended for use in a calibration hierarchy, in the validation of measurement procedures, or as quality control material for the determination of albumin, creatinine, or the albumin-to-creatinine ratio (ACR) in human urine. A unit of SRM 3666 consists of four (4) vials of frozen pooled human urine with four (4) different levels (Level I to Level IV) of endogenous albumin determined using the NIST candidate reference measurement procedure (RMP) for albumin in urine; and creatinine determined using a modification of the NIST RMP for creatinine in serum. The certified values and associated uncertainties for albumin and creatinine were used to calculate the ACR for each level (Level I to Level IV). This publication documents the production, measurement processes, results, and statistical evaluations involved in the production and certification of SRM 3666.

Citation

Special Publication (NIST SP) - 260-238-upd2

Report Number

260-238-upd2

NIST Pub Series

Special Publication (NIST SP)

Pub Type

NIST Pubs

Keywords

Albumin, Albumin-to-Creatinine Ratio (ACR), Creatinine, Human Urine, Reference Measurement Procedure (RMP), Standard Reference Material (SRM)

Citation

Green, A. , Camara, J. and Heckert, N. (2025), Certification of Standard Reference Material® 3666: Albumin and Creatinine in Frozen Human Urine - INCLUDES UPDATES AS OF 07-02-2025, Special Publication (NIST SP), National Institute of Standards and Technology, Gaithersburg, MD (Accessed September 26, 2025)