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In Vivo Characteristics of Premixed Calcium Phosphate Cements When Implanted in Subcutaneous Tissues and Periodontal Bone Defects



Akiyoshi Sugawara, Kenji Fujikawa, Satoshi Hirayama, Shozo Takagi, Laurence Chow


The present study evaluated characteristics of premixed calcium phosphate cements (Pre-CPCs) when implanted in subcutaneous tissues (Study I) and used for repairing surgically created two-wall periodontal defects (Study II). Pre-CPC pastes were prepared by combining CPC powders that consisted of CPC-1: Ca4(PO4)2O and CaHPO4, CPC-2: α-Ca3(PO4)2 and CaCO3 or CPC-3: DCPA and Ca(OH)2 with a glycerol at powder-to-liquid mass ratios of 3.5, 2.5, and 2.5, respectively. In each cement mixture, the Ca to P molar ratio was 1.67. The glycerol contained Na2HPO4 (30 mass %) and hydroxypropyl methylcellulose (0.55 %) to accelerate cement hardening and improve washout resistance, respectively. In Study I, the test materials were implanted subcutaneously in rats. Four weeks after the operation, the animals were sacrificed and histopathological observations were performed. The results showed that all of the implanted materials exhibited very slight or negligible inflammatory reactions in tissues contacted with the implants. In Study II, The dogs were sacrificed, and biopsies of the filled defect were obtained at 1, 3 and 6 months after surgery. Results showed that one month after surgery, the implanted Pre-CPC-1 paste was partially replaced by bone and was converted to bone in 6 months. The pockets filled with Pre-CPC-2 were completely covered by newly formed bone in 1 month. The Pre-CPC-2 was partially replaced by trabecular bone in 1 month and was completely replaced by bone in 6 months. Examination of 1 month and 3 month samples indicated that Pre-CPC-2 resorbed and was replaced by bone more rapidly than Pre-CPC-1. Both Pre-CPC pastes were highly osteoconductive. When implanted in periodontal defects, Pre-CPC-2 was replaced by bone more rapidly than Pre-CPC-1.
Journal of Research (NIST JRES) -


Premixed calcium phosphate cement, animal study, periodontal bone defects, histopathological examination, new bone formation


Sugawara, A. , Fujikawa, K. , Hirayama, S. , Takagi, S. and Chow, L. (2010), In Vivo Characteristics of Premixed Calcium Phosphate Cements When Implanted in Subcutaneous Tissues and Periodontal Bone Defects, Journal of Research (NIST JRES), National Institute of Standards and Technology, Gaithersburg, MD, [online], (Accessed May 23, 2024)


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Created July 31, 2010, Updated October 12, 2021