In Vitro Biocompatibility of Hydrolytically Degraded Poly(d,l-lactic acid)

Published

January 1, 2004

Author(s)

S Yoneda, William F. Guthrie, David S. Bright, C A. Khatri, Francis W. Wang

Abstract

n order to investigate the effects of hydrolytic degradation on the biocompatibility of poly(d,l-lactic acid) [P(d,l-LA)], the initial attachment of MC3T3-E1 osteoblast-like cells on various degraded P(d,l-LA) disks was assessed. MC3T3-E1 cells were seeded on P(d,l-LA) disks (10 mm in diameter and 1.65 mm in thickness) that had been degraded by immersion in a hydrolyzing medium for (0 to 4) weeks. The cell-spread area was measured with a fluorescence microscope after staining the plasma membrane with a fluorescent dye. The focal adhesions of the cells were also investigated by immunofluorescence staining of vinculin. The cell-spread area of the cells on P(d,l-LA) disks that were not degraded did not differ significantly from that of the cells on tissue-culture polystyrene, but the degradation of P(d,l-LA) disks affected cell spreading.The cell-spread area decreased linearly with the degradation time of the disks at a rate of (-741 307) mm2/week (all uncertainties quoted are expanded uncertainties at the 95% confidence level). Compared with the cells on non-degraded P(d,l-LA) disks, the cells on P(d,l-LA) disks that had been degraded for 4 weeks also showed irregular morphologies. Focal adhesions began to disappear for the cells on P(d,l-LA) disks degraded for one week. The number of the live cells [up to (2.099 0.268) cells/mm2 in log10 units, depending on the measurement location within the samples] on P(d,l-LA) disks also decreased linearly with the degradation time of the disks at a rate of up to (-0.175 0.064) (cells/mm2)/week in log10 units, again depending on the measurement location within the samples. The dehydrogenase activity, as measured by the WST-1 assay, also significantly decreased with the degradation time of the P(d,l-LA) disks

Citation

World Biomaterials Congress

Volume

109(2)

Pub Type

Journals

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Keywords

focal adhesion, hydrolytic degradation, poly(d, l-lactide)

Citation

Yoneda, S. , Guthrie, W. , Bright, D. , Khatri, C. and Wang, F. (2004), In Vitro Biocompatibility of Hydrolytically Degraded Poly(d,l-lactic acid), World Biomaterials Congress, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=852261 (Accessed November 4, 2025)