Laesecke, A.
and Burger, J.
(2014),
Viscosity Measurements of DNA Solutions with and without Condensing Agents, Biorheology, [online], https://doi.org/10.3233/BIR-140649
(Accessed December 1, 2024)
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Viscosity Measurements of DNA Solutions with and without Condensing Agents
Published
Author(s)
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Abstract
Aqueous DNA solutions at three concentrations were studied as model sputum by viscosity measurements at ambient pressure. Initial measurements at 25 °C, 37.78 °C, and 50 °C indicated a viscosity increase toward 50 °C. This phenomenon was studied by extended measurements from 10 °C to 90 °C, which revealed concentration-dependent viscosity maxima in the range of 50 °C to 65 °C. The associated viscosity increase were up to 400%. A two-liquids model for solutions of double-and single-stranded DNA is developed that represents the experimental data closely. Three polycations were tested as viscosity-reducing agents with measurements from 25 °C to 50 °C. The most significant viscosity reduction was achieved with spermine in a solution with mg/ml DNA concentration.Citation
Biorheology
Volume
51
Pub Type
Journals
Download Paper
Keywords
Calf thymus DNA, capillary viscometer, cystic fibrosis, rotating concentric cylinder viscometer, sputum, viscosity
Citation
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Created July 7, 2014, Updated November 10, 2018