Value Assignment of Vitamin D Metabolites in Vitamin D Standardization Program (VDSP) Serum Samples

Published

June 16, 2017

Author(s)

Karen W. Phinney, Johanna Camara, Susan Tai, Lane C. Sander, Stephen A. Wise, Linde A. De Grande, Linda M. Thienpont, Antonio M. Possolo, Blaza Toman, Christopher T. Sempos, J. M. Betz, Paul M. Coates

Abstract

Assay variability has been cited as an obstacle to establishing optimal vitamin D exposure. As part of the Vitamin D Standardization Program (VDSP) effort to standardize the measurement of total 25(OH)D, value assignment of total 25(OH)D in 50 single donor serum samples was performed using two isotope-dilution LC-MS/MS methods. Both methods are recognized as reference measurement procedures (RMPs). These samples and their assigned values serve as the foundation for several aspects of the VDSP. To our knowledge, this is the first time that two RMPs have been used to assign values to such a large number of serum samples.

Citation

Journal of Aoac International

Volume

100

Issue

5

Pub Type

Journals

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DOI Link

Keywords

reference measurement procedure, standardization, vitamin D

Citation

Phinney, K. , Camara, J. , Tai, S. , Sander, L. , Wise, S. , De, L. , Thienpont, L. , Possolo, A. , Toman, B. , Sempos, C. , Betz, J. and Coates, P. (2017), Value Assignment of Vitamin D Metabolites in Vitamin D Standardization Program (VDSP) Serum Samples, Journal of Aoac International, [online], https://doi.org/10.5740/jaoacint.17-0204 (Accessed October 28, 2025)