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Triglycidylamine Crosslinking of Porcine Aortic Valve Cusps or Bovine Pericardium Results in Improved Biocompatibility, Biomechanics and Calcification Resistance - Chemical and Biological Mechanisms

Published

Author(s)

J Connolly, E Alferiev, J N. Clark, Naomi Eidelman, M Sacks, E Palmatory, A Kronsteinter, S DeFelice, J Xu, R Ohri, N Navneet, N Vyavahare, R J. Levy

Abstract

We investigated a novel polyepoxide crosslinker that was hypothesized to confer both material stabilization and calcification resistance when used to prepare bioprosthetic heart valves. Triglycidylamine (TGA) was synthesized via reacting epichlorhydrin and NH3. TGA was used to crosslink porcine aortic cusps, bovine pericardium, and type I collagen. Control materials were crosslinked with glutaraldehyde (Glut). Results: TGA-pretreated materials had shrink temperatures comparable to Glut fixation. However, TGA crosslinking conferred significantly greater collagenase resistance than Glut-pretreatment, and significantly improved biomechanical compliance. Sheep aortic valve interstitial cells (SAVIC) grown on TGA-pretreated collagen did not calcify, while SAVIC grown on control substrates calcified extensively.Rat subdermal implants (porcine aortic cusps/bovine pericardium) pretreated with TGA demonstrated significantly less calcification than Glut-pretreated. Investigations of extracellular matrix (ECM) proteins associated with calcification, matrix metalloproteinases 2&9 (MMP2&MMP9), tenascin-C (TNC), and osteopontin (OPN), revealed that MMP9 and TNC demonstrated reduced expression both in vitro and in vivo with TGA crosslinking compared to controls, while OPN and MMP2 expression were not affected. Conclusions: TGA-pretreatment of heterograft biomaterials results in improved stability compared to Glut, confers biomechanical properties superior to Glut-crosslinking, and demonstrates significant calcification resistance.
Citation
American Journal of Pathology
Volume
166
Issue
No. 1

Keywords

biocompatibility, biomechanics, bovine pericardium, calcification, FTIR microspectroscopy, porcine aortic valve cusps, Triglycidyl

Citation

Connolly, J. , Alferiev, E. , Clark, J. , Eidelman, N. , Sacks, M. , Palmatory, E. , Kronsteinter, A. , DeFelice, S. , Xu, J. , Ohri, R. , Navneet, N. , Vyavahare, N. and Levy, R. (2005), Triglycidylamine Crosslinking of Porcine Aortic Valve Cusps or Bovine Pericardium Results in Improved Biocompatibility, Biomechanics and Calcification Resistance - Chemical and Biological Mechanisms, American Journal of Pathology (Accessed February 26, 2024)
Created December 31, 2004, Updated October 12, 2021