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Tools and Approaches for the Assessment of Nanomaterial Induced Oxidative DNA Damage

Published

Author(s)

Elijah J. Petersen, Bryce J. Marquis, Pawel Jaruga, M Miral Dizdar, Bryant C. Nelson

Abstract

Hyphenated mass spectrometry techniques have been employed as one of the primary analytical tools for investigating the effects of ionizing radiation, chemical/biological carcinogens, and oxygen derived free radicals on the induction and subsequent repair of oxidatively-induced DNA damage (DNA lesions) in living systems. The National Institute of Standards and Technology has established a comprehensive research program focused on identifying mechanisms of DNA damage caused by commercially relevant engineered nanoparticles (NPs) using these techniques for the quantification of oxidatively-induced DNA damage. We present an overview of our recent findings from studies on metal (gold) and metal oxide (ultrafine superparamagnetic iron oxide) nanoparticles using isotope dilution liquid chromatography and gas chromatography/mass spectrometry analysis, respectively.
Proceedings Title
Nanotech 2012
Conference Dates
June 18-21, 2012
Conference Location
Santa Clara, CA

Keywords

nanotoxicology, Comet assay, genotoxicity, biomarker, toxicology

Citation

Petersen, E. , Marquis, B. , Jaruga, P. , , M. and Nelson, B. (2012), Tools and Approaches for the Assessment of Nanomaterial Induced Oxidative DNA Damage, Nanotech 2012, Santa Clara, CA, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=911145 (Accessed April 23, 2024)
Created June 20, 2012, Updated February 19, 2017