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Thermally-Treated Clay as a Stationary Phase in Liquid Chromatography



K E. Miller, Thomas J. Bruno


Spray-dried, spherical synthetic hectorite particles are thermally-treated andised as adsorbent materials in reversed-phase high pressure liquid chromatography. The retention of a series of mono- and di-substituted aromatimompounds is evaluated to study the retention mechanisms on the clay mineralRetention of solutes on thermally-treated clays is markedly different than that measured on octadecyl silica (ODS) column under identical conditions, but remarkably similar to retention of the same solutes on porous graphitic carbor-columns. The clay columns also have enhanced selectivity over the ODS column in the separation of positional isomers of dinitrobenzene. The proposed retenibn mechanism is governed by 2 combination of attractive dispersiveforces aswell as dipole-induced dipole interactions between the surface of the clay ancthe aromatic solutes.
Journal of Chromatography A
No. 1-2


adsorption, clay, HPLC, laponite-rd, positional isomers, thermal treatment


Miller, K. and Bruno, T. (2004), Thermally-Treated Clay as a Stationary Phase in Liquid Chromatography, Journal of Chromatography A (Accessed May 18, 2024)


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Created June 30, 2004, Updated October 12, 2021