Theory for Polymer Analysis Using Nanopore-based Single-molecule Mass Spectrometry
Joseph E. Reiner, John J. Kasianowicz, Brian J. Nablo, Joseph W. Robertson
Nanometer-scale pores have demonstrated potential as a tool for the electrical detection and characterization of molecules. To provide a physical basis for nanopore-based analytical metrology, we show that a simple model quantitatively describes how one type of analyte (polymers of poly(ethylene glycol), PEG) reduces the ionic current in a single α-hemolysin ion channel. Two critical observations are apparent in the data. The depth of PEG-induced current blockades and the residence time of the polymer in the nanopore both decrease with increasing applied potential. A model is presented which describes these phenomena by analyzing the polymer size, and cation interactions with the polymer under moderate confinement.
Proceedings of the National Academy of Sciences of the United States of America
, Kasianowicz, J.
, Nablo, B.
and Robertson, J.
Theory for Polymer Analysis Using Nanopore-based Single-molecule Mass Spectrometry, Proceedings of the National Academy of Sciences of the United States of America, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=904049
(Accessed December 3, 2023)