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Surface Plasmon Resonance Measurements of DNA Probe/Target Hybridization Reactions on Gold Surfaces

Published

Author(s)

G B. Saupe, Michael J. Tarlov

Abstract

Optimizing and understanding the parameters involved in thin film DNA hybridizations are important to emerging DNA sensor array technologies. We recently constructed a Surface Plasmon Resonance (SPR) instrument and software for measuring the small refractive index changes, and hence mass changes, that are involved during monolayer DNA hybridization events. A model system of DNA attached to gold surfaces has been developed for this work and is ideallysuited for study by SPR techniques. Short sequences of single-stranded DNA, so-called ssDNA probes, are end-tethered to gold surfaces through a thiol linker. Complementary ssDNA targets in solution can hybridized to the surface bound probes. We are studying how variations in probe surface structure and sequence of ssDNA target affect hybridization rates and efficiencies.
Proceedings Title
Sigma Xi Post Doctoral Poster Presentations, 2000
Conference Dates
February 17-18, 2000
Conference Title
Sigma Xi Web Page

Keywords

DNA hybridizations, DNA sensor array technologies, Surface Plasmon Resonance, thin film DNA hybridizations

Citation

Saupe, G. and Tarlov, M. (2000), Surface Plasmon Resonance Measurements of DNA Probe/Target Hybridization Reactions on Gold Surfaces, Sigma Xi Post Doctoral Poster Presentations, 2000 (Accessed March 2, 2024)
Created February 1, 2000, Updated February 17, 2017