Standard Practice for Determining the Relative Spectral Correction Factors for the Emission Signal of Fluorescence Spectrometers

Published

August 15, 2013

Author(s)

Paul C. DeRose

Abstract

This standard practice is intended to aid in the calibration and qualification of fluorescence spectrometers as part of complying with regulatory and other QA/QC requirements. It specifies the materials and procedures used in three different methods for the correction of the relative responsivity of the instrument's detection system as a function of wavelength, also known as relative spectral correction. ASTM Luminescence subcommittee E13.01.01 intends to use this document as a template for a similar ASTM guide.

Citation

NIST Interagency/Internal Report (NISTIR) - 7915

Report Number

7915

NIST Pub Series

NIST Interagency/Internal Report (NISTIR)

Pub Type

NIST Pubs

Download Paper

https://doi.org/10.6028/NIST.IR.7915

Keywords

biosystems and health, calibration, fluorescence, instrument qualification, method validation, reference materials, spectral correction

Citation

DeRose, P. (2013), Standard Practice for Determining the Relative Spectral Correction Factors for the Emission Signal of Fluorescence Spectrometers, NIST Interagency/Internal Report (NISTIR), National Institute of Standards and Technology, Gaithersburg, MD, [online], https://doi.org/10.6028/NIST.IR.7915 (Accessed October 7, 2025)