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Standard Guide to Fluorescence - Instrument Calibration and Validation



Paul C. DeRose


This guide is intended to aid in the calibration, qualification and performance verification of fluorescence-based instrumentation as part of complying with regulatory and other QA/QC requirements. Commonly-used, commercially available materials and methods are listed for each type of calibration or correction for fluorescence instruments under the appropriate subsection (spectral emission correction, wavelength accuracy, etc.) A general description, the level of quality, precision and accuracy attainable, caveats, and useful references are given for each entry. Precision and accuracy or uncertainty are also given, when applicable, and are approximated in cases where these values have not been well established. ASTM Luminescence subcommittee E13.01.01 intends to use this document as a template for a similar ASTM guide.
NIST Interagency/Internal Report (NISTIR) - 7458
Report Number


biosystems and health, calibration, fluorescence, instrument qualification, method validation, reference materials


DeRose, P. (2008), Standard Guide to Fluorescence - Instrument Calibration and Validation, NIST Interagency/Internal Report (NISTIR), National Institute of Standards and Technology, Gaithersburg, MD, [online], (Accessed June 19, 2024)


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Created January 2, 2008, Updated November 10, 2018