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Stability of Bisphenol A, Triethylene-Glycol Dimethacrylate, and Bisphenol A Dimethacryate in Whole Saliva



J C. Atkinson, F Diamond, F Eichmiller, R Selwitz, Gordon L. Jones


Objectives: This study investigated the stability of compounds of dental sealant materials in a salivary matrix.Methods: Various amounts of bisphenol A (BPA), bisphenol A dimethacrylate (BIS-DMA) and triethylene-glycol dimethacrylate (TEGDMA) were added to whole salivary samples, and stored at -70 C or -20 C for up to 4 months. In other experiments, four separate whole salivary or water samples with BIS-DMA (200 ng/ml) were incubated for 0,1,2,4 or 24 h at 37 C. Levels of analytes were determined by capillary gas chromatography / mass spectrophotometry (GC/MS) and high-performance liquid chromatography (HPLC).Results: BPA was stable under all tested conditions. Samples originally containing BIS-DMA had high levels of BPA and almost no BIS-DMA after four months at -20 C. Salivary samples incubated at 37 C originally containing only BIS-DMA (200 ng/mL) demonstrated rapid decreases of BIS-DMA and increases of BPA. By 24 h, the mean BIS-DMA concentration fell to 21.8 (25) ng/mL, while BPA increased to 100 (48) ng/mL. Only slight decreases in BIS-DMA and no BPA were present in the water samples incubated at 37 C. BPA, BIS-DMA, and TEGDMA were stable if salivary samples were stored at -70 C. Acidification of salivary samples prevented the breakdown of BIS-DMA.Significance: BIS-DMA is converted rapidly to BPA in the presence of whole saliva. This could account for the findings of BPA in clinical samples collected after the placement of certain sealant products. Lowering salivary pH and temperature can slow this process and this method should be used for clinical studies of salivary BPA leached from restorative materials.
Dental Materials Congress
No. 2


bisphenol A, bisphenol A dimethacrylate, dental material, dental sealant saliva


Atkinson, J. , Diamond, F. , Eichmiller, F. , Selwitz, R. and Jones, G. (2002), Stability of Bisphenol A, Triethylene-Glycol Dimethacrylate, and Bisphenol A Dimethacryate in Whole Saliva, Dental Materials Congress (Accessed June 25, 2024)


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Created February 28, 2002, Updated October 12, 2021