Gallagher, D.
, Godoy-Ruiz, R.
, Krejcirikova, A.
and Tugarinov, V.
(2011),
Solution NMR Evidence for Symmetry in Functionally or Crys-tallographically Asymmetric Homodimers, Journal of the American Chemical Society, [online], https://doi.org/10.1021/ja206967d
(Accessed April 26, 2024)
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Solution NMR Evidence for Symmetry in Functionally or Crys-tallographically Asymmetric Homodimers
Published
Author(s)
, Raquel Godoy-Ruiz, Anna Krejcirikova, Vitali Tugarinov
Abstract
A recurrent theme of many structural studies of homo-oligomeric protein systems is concerned with verification that the conformation observed in a crystal repre¬sents the functionally relevant structure. An asymmetric con-formation adopted by two chemically identical subunits in homo-oligomers can represent an intrinsic property of a protein or be an artifact induced by crystal packing forces. Solution NMR studies can distinguish between these two possibilities. Using methyl-based NMR spectroscopy we provide evidence for symmetry in the absence of ligands in several homodimeric proteins that are either asymmetric functionally and/or adopt different conformations of the two subunits in available x-ray structures.Citation
Journal of the American Chemical Society
Volume
133
Issue
49
Pub Type
Journals
Download Paper
Keywords
NMR, CRP, Symmetry, Crystallography
Citation
Created October 18, 2011, Updated March 14, 2023