Solid-Phase ATRP Synthesis of Peptide-Polymer Hybrids
Ying Mei, Kathryn L. Beers, H M. Byrd, David L. VanderHart, N. R. Washburn
A versatile methodology for preparing hybrid biomaterials by atom transfer radical polymerization from resin-supported peptides has been established. As an example, we have synthesized a GRGDS-functionalized poly(2-hydroxyethyl methacrylate). The peptide-polymer was characterized by 1H NMR and GPC and found to have a number average molecular weight of 3900 and a polydispersity of 1.47. These values are comparable to those obtained from solution-phase syntheses, suggesting it is possible to retain significant control over the polymerization from a peptide-conjugated solid support. Solid state 13C NMR was used to characterize multiple steps in the reaction and the synthesis was found to go to near-completion. We have performed cell adhesion experiments and observed the GRGDS sequence promoted cell adhesion.
, Beers, K.
, Byrd, H.
, VanderHart, D.
and Washburn, N.
Solid-Phase ATRP Synthesis of Peptide-Polymer Hybrids, Journal of the American Chemical Society, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=852269
(Accessed July 31, 2021)