In situ monitoring of enzyme-catalyzed (co)polymerizations by Raman spectroscopy
Matthew T. Hunley, Atul Bhangale, Santanu S. Kundu, Peter M. Johnson, Michael S. Waters, Richard Gross, Kathryn Beers
In situ, fiber optic-based Raman spectroscopy provided real time monitoring of enzyme-catalyzed ring-opening homo- and copolymerizations of ε-caprolactone (ε-CL) and δ-valerolactone (δ-VL). A custom designed reactor equipped with in situ fiber optic probe was used to measure monomer conversion as a function of time. The results from the in situ technique were in good agreement with those determined by offline 1H NMR analysis. Monomer reactivity ratios for the lipase-catalyzed copolymerization of ε-CL and δ-VL were estimated using the Kelen-Tudos method as rε-CL = 0.38 and rδ-VL = 0.29.
, Bhangale, A.
, Kundu, S.
, Johnson, P.
, Waters, M.
, Gross, R.
and Beers, K.
In situ monitoring of enzyme-catalyzed (co)polymerizations by Raman spectroscopy, Polymer Chemistry, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=909434
(Accessed September 25, 2023)