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In situ monitoring of enzyme-catalyzed (co)polymerizations by Raman spectroscopy



Matthew T. Hunley, Atul Bhangale, Santanu S. Kundu, Peter M. Johnson, Michael S. Waters, Richard Gross, Kathryn Beers


In situ, fiber optic-based Raman spectroscopy provided real time monitoring of enzyme-catalyzed ring-opening homo- and copolymerizations of ε-caprolactone (ε-CL) and δ-valerolactone (δ-VL). A custom designed reactor equipped with in situ fiber optic probe was used to measure monomer conversion as a function of time. The results from the in situ technique were in good agreement with those determined by offline 1H NMR analysis. Monomer reactivity ratios for the lipase-catalyzed copolymerization of ε-CL and δ-VL were estimated using the Kelen-Tudos method as rε-CL = 0.38 and rδ-VL = 0.29.
Polymer Chemistry


Raman spectroscopy, enzyme-catalyzed polymerization, ring-opening polymerization, copolymerization, reactivity ratios


Hunley, M. , Bhangale, A. , Kundu, S. , Johnson, P. , Waters, M. , Gross, R. and Beers, K. (2011), In situ monitoring of enzyme-catalyzed (co)polymerizations by Raman spectroscopy, Polymer Chemistry, [online], (Accessed May 25, 2024)


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Created November 9, 2011, Updated October 12, 2021