Hromada, L.
, Nablo, B.
, Gaitan, M.
, Kasianowicz, J.
and DeVoe, D.
(2008),
Single molecule measurements within individual membrane-bound ion channels using a polymer-based bilayer lipid membrane chip, Lab on A Chip, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=32821
(Accessed October 8, 2025)
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Single molecule measurements within individual membrane-bound ion channels using a polymer-based bilayer lipid membrane chip
Published
Author(s)
Louis P. Hromada, , , , Don DeVoe
Abstract
A polymer microfluidic platform for electrophysiological ion channel measurements is presented. The system includes an open-well bilayer lipid membrane formation site, integrated Ag/AgCl reference electrodes for on-chip electrical measurements, and multiple microchannels for independent ion channel and analyte delivery. Details of chip fabrication, bilayer membrane formation, and alpha-hemolysin ion channel incorporation are discussed. Using this integrated platform, measurements of interactions between membrane-bound ion channels and single poly(ethylene glycol) molecules are demonstrated.Citation
Lab on A Chip
Pub Type
Journals
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Keywords
electrophysiology, ion channel, microfluidics, on-chip, poly(ethylene glycol), single molecule
Citation
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Created February 28, 2008, Updated October 12, 2021