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PUBLICATIONS

Single molecule measurements within individual membrane-bound ion channels using a polymer-based bilayer lipid membrane chip

Published

Author(s)

Louis P. Hromada, Brian J. Nablo, Michael Gaitan, John J. Kasianowicz, Don DeVoe

Abstract

A polymer microfluidic platform for electrophysiological ion channel measurements is presented. The system includes an open-well bilayer lipid membrane formation site, integrated Ag/AgCl reference electrodes for on-chip electrical measurements, and multiple microchannels for independent ion channel and analyte delivery. Details of chip fabrication, bilayer membrane formation, and alpha-hemolysin ion channel incorporation are discussed. Using this integrated platform, measurements of interactions between membrane-bound ion channels and single poly(ethylene glycol) molecules are demonstrated.
Citation
Lab on A Chip
Pub Type
Journals

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Keywords

electrophysiology, ion channel, microfluidics, on-chip, poly(ethylene glycol), single molecule
Nanobiotechnology

Citation

Hromada, L. , Nablo, B. , Gaitan, M. , Kasianowicz, J. and DeVoe, D. (2008), Single molecule measurements within individual membrane-bound ion channels using a polymer-based bilayer lipid membrane chip, Lab on A Chip, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=32821 (Accessed September 8, 2024)

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Created February 28, 2008, Updated October 12, 2021