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Single Molecule Analysis of Electrodynamically Stretched DNA in Nanochannels

Published

Author(s)

Samuel M. Stavis, Christian H. Reccius, John T. Mannion, Larry P. Walker, Harold G. Craighead

Abstract

A rapid (~20 ms) and precise (~110 nm) analytical method is presented for stretching and interrogating single DNA molecules. Conformation, length, speed and fluorescence intensity measurements are obtained simultaneously. This confluence is a step forward towards applications that place a premium on analysis time and spatial resolution, such as single molecule genomic sequencing. Several physical aspects of the electrophoretic translocation of DNA through a nanochannel are investigated.
Proceedings Title
Proceedings of the 12 International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS 2008)
Conference Dates
October 12-16, 2008
Conference Location
San Diego, CA
Conference Title
12 International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS 2008)

Keywords

Nanochannel, Nanofluidic, DNA, Single Molecule

Citation

Stavis, S. , Reccius, C. , Mannion, J. , Walker, L. and Craighead, H. (2008), Single Molecule Analysis of Electrodynamically Stretched DNA in Nanochannels, Proceedings of the 12 International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS 2008), San Diego, CA (Accessed July 22, 2024)

Issues

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Created October 13, 2008, Updated February 17, 2017