Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Single Molecule Analysis of Electrodynamically Stretched DNA in Nanochannels

Published

Author(s)

Samuel M. Stavis, Christian H. Reccius, John T. Mannion, Larry P. Walker, Harold G. Craighead

Abstract

A rapid (~20 ms) and precise (~110 nm) analytical method is presented for stretching and interrogating single DNA molecules. Conformation, length, speed and fluorescence intensity measurements are obtained simultaneously. This confluence is a step forward towards applications that place a premium on analysis time and spatial resolution, such as single molecule genomic sequencing. Several physical aspects of the electrophoretic translocation of DNA through a nanochannel are investigated.
Proceedings Title
Proceedings of the 12 International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS 2008)
Conference Dates
October 12-16, 2008
Conference Location
San Diego, CA
Conference Title
12 International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS 2008)

Keywords

Nanochannel, Nanofluidic, DNA, Single Molecule

Citation

Stavis, S. , Reccius, C. , Mannion, J. , Walker, L. and Craighead, H. (2008), Single Molecule Analysis of Electrodynamically Stretched DNA in Nanochannels, Proceedings of the 12 International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS 2008), San Diego, CA (Accessed November 3, 2024)

Issues

If you have any questions about this publication or are having problems accessing it, please contact reflib@nist.gov.

Created October 13, 2008, Updated February 17, 2017